KLONOWANIE DNA PDF

Blog. 18 December Prezi Awards The best presentations have arrived. 5 December Do this, not that: Keynote speech. 28 November Wady i Zalety Klonowania Idea 1. Idea 2. Rozmnażanie bezpłciowe – naturalne klonowanie. Klonowanie zachodzi przez: Klonowanie DNA. KLONOWANIE Klony DNA służą do: Co to jest klonowanie? Klonowanie – tworzenie genetycznej kopii fragmentu DNA, komórki lub organizmu.

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A bunch of E.

Cut open the plasmid and “paste” in the gene. The basic answer klonownie that a heat shock makes the bacterial membrane more permeable to DNA molecules, such as plasmids.

Because of these possibilities, it’s important to collect plasmid DNA from each colony and check to see if it matches the plasmid we were trying to build. Although, as we’ll see, it’s still quite fascinating. So this one is a good colony, put a checkmark there.

It’s identical copies of a piece of DNA. So let me, we’re pasting dja into the plasmid. And an organism that’s typically used, or a type of organism is bacteria and E. The purified protein can klonpwanie used for experiments or, in the case of insulin, administered to patients.

This is not a useful plasmid. And usually it’s a piece of DNA that codes for something we care about, it is a gene that will express itself as a protein that we think is useful in some way.

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And this is amazing because obviously DNA, this isn’t stuff that we can, you know, xna with our hands the way that we would copy and paste things with tape.

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Enzymy restrykcyjne i ligaza DNA. Insert the plasmid into bacteria. Suppose that we identify a colony with a “good” plasmid.

Or it can even express itself just like the genes of the organism that are in the chromosomes, express themselves. A backwards gene cannot be expressed in bacteria to make a protein.

KLONOWANIE by KurkaxD KurkaxD on Prezi

They’re not even going to grow because there’s antibiotics mixed in with those nutrients. The bacteria can then be lysed split open to release the protein. And in order for them to fit there’s oftentimes these overhangs over here. Cells that have produced protein are burst open lysedreleasing the protein and the other cell contents. And so what you then do is you place the solution that has your bacteria, some of which will have taken up the plasmid, and you put it and then you try to grow the bacteria on a plate.

However, all it means to clone something is to make a genetically exact copy of it. See the article on restriction enzymes and DNA ligase for a more concrete example of how and why these different ligation products can form. This is the desired plasmid from the ligation.

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And so just like that you can take this, you can take this colony right over here, and put it into another solution or continue to grow it and you will have multiple copies of that gene that are inside of that bacteria. This step uses restriction enzymes and DNA ligase and is called a ligation. So you don’t want that one.

Selekcja i transformacja u bakterii (artykuł) | Khan Academy

And so you won’t know, hey when this bacteria, when it keeps replicating it might form one of these, it might form one of these colonies. In the final step, the protein of interest is released from the column and collected for use.

Selekcja i transformacja u bakterii. Colonies with the right plasmid can be grown to make large cultures of identical bacteria, which are used to produce plasmid or make protein. But these are not going to survive.

The promoter “points” towards the right, meaning that it will drive transcription of the DNA sequence that lies to the right. Transformation and selection of bacteria are key steps in DNA cloning. Because of this, the target protein must be purifiedor separated from the other contents of xna cells by biochemical techniques.