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Immunohistochemical stainingalso immunostainingis a powerful tool. It is abbreviated IHC. Method was introduced in by Coons. It is touched upon the in the quality article in the immunohistochemistry section.

There are at least 62 pre-analytical variables to be considered, that may affect staining results. Generally, interpretations can be subjective, and this is especially true when the staining is weak and focal. In other words, ” The cynical might say it is an unwritten rule that: In cases where the morphology is unclear, it is judicious to have two or more immunostains that support the diagnosis, and negative stains for important entities in the differential diagnosis.


E-immunohistochemistry : Immunohistochemistry vade mecum

Publications with contradicting results are not uncommon. Differences can arise from the fixation, processing protocol, antibody clone and interpretation.

According to Galloway, one third pathologists substantially overestimate immunihistochemistry diagnostic significance of unexpected immunohistochemical staining results. Are typically seen in carcinomas.

Intrahepatic cholangiocarcinoma ICC vs. This is inmunohistochemistry more extensively in the lymphoma immunohistochemiwtry. This is covered more extensively in the Hodgkin lymphoma article. Ann N Y Acad Sci Appl Immunohistochem Mol Morphol 15 1: Appl Immunohistochem Mol Morphol 22 4: Arch Pathol Lab Med 5: Patholog Res Int J Dermatol Sci 31 3: Am J Dermatopathol 30 2: Arch Pathol Lab Med 1: Langenbecks Arch Chir 3: Hum Pathol 24 3: Immunohistochemical and immunoblot studies.

Am J Surg Pathol 15 8: Mod Pathol 17 Am J Clin Pathol. Percentage of positivity and distribution is affected by the cut-point threshold”. A Volume in the Pattern Recognition series 1st ed.

Immunohistochemistry vade mecum

Zhonghua Zhong Liu Za Zhi. Am J Surg Pathol 32 Int J Gynecol Pathol 27 4: The Washington Manual of Surgical Pathology 1st ed. Diagnostic Criteria Handbook in Histopathology: A Surgical Pathology Vade Mecum 1st ed.


J BUON 17 1: World J Surg Oncol 8: Retrieved from ” https: Navigation menu Personal tools Log in. Views Read View source View history. Navigation Main page Recent changes Random page Help. This page was last modified on 2 Julyat